Skip to content

Of Mice and Microscopes

Share on facebook
Share on twitter
Share on linkedin

The Olympus Fluoview FV1000-MPE using ultrashort pulsed IR laser


  • Peak power at 800nm: 312.5kW
  • Average power at 800nm: > 2.5W
  • Pulse repetition: 80MHz
  • Pulse width: < 100fs
  • Tuning range: 710-1040nm
  • Cost: €0.82 Million

In the last 25 years, two-photon excitation microscopy paved the road for the most significant advance in bio-imaging. This year, four scientists (Winfried Denk, Arthur Konnerth, Karel Svoboda, and David Tank) were awarded the prestigious Grete Lundbeck European Brain Research prize for its invention and development. The method has transformed brain research since it allows real-time examination of the brain’s finest structures. It is powerfully used to investigate stroke, Alzheimer’s disease, migraine, and epilepsy.

The University of Malta’s microscope combines ultrashort-pulsed infrared laser to excite fluorescent molecules up to a depth of 1mm in the rodent brain. The technique allows flexible detection of the brain’s geometries and can look 5–20 times deeper than other types of fluorescent microscopes. The customised setup can perform live imaging to create 3D brain images.

At the University the instrument is used by six scientists on a daily basis with two foreign collaborators in fields which include the evolution of stroke, brain-blood flow dynamics, neurovascular coupling, epilepsy, potassium channel physiology, and white matter injury.

The microscope can aquire images through four simultaneous color channels at 30 frames per second. During imaging of small anaesthesized animals, the microscope is equipped for monitoring vital signs. The instrument is housed in a temperature of 22°C and <30% humidity controlled environment adjacent to a surgical preparation suite and imaging workstation. Two-photon microendoscopy has started to find clinical applications in cancer. There are ongoing developments to image deeper brain structures.


More to Explore

Food Security in Malta: Is it Possible?

It is unlikely that Malta can ever become self-sufficient when it comes to food. The next best thing for the island is to guarantee sustainable food security. THINK sits with 4 experts to see how this could be possible.

Il-Baqra Tinbieh Kollha: A Journey Towards Abattoir Self-Sufficiency and Sustainability

While plant-based food is gaining popularity, the majority of the world’s population still consumes meat. “Il-baqra tinbieħ kollha” (every part of the cow can be sold), a Maltese saying, emphasises that every part of harvested livestock is used. It is imperative that the meat industry develop sustainable practices to reduce its environmental impact, such as utilising every part of the slaughtered animal.

Preserving the Past: UM Library’s Digitisation of Centuries-old Pamphlets

The University of Malta’s Library has diligently digitised historical pamphlets, some dating as far back as the 1600s, allowing students and researchers to explore battles, cultural shifts, and more through primary sources. THINK speaks with library manager Antida Mizzi to explore the commitment to safeguarding history, making it accessible for researchers and scholars dedicated to unearthing the stories that define us.

No comment yet, add your voice below!

Add a Comment